CyFlow™ CD1c PE

CyFlow™ CD1c PE
Antigen: CD1c
Alternative Name: CD1, R7
Clone: L161
Application: Flow cytometry
Format/Fluorochrome: PE
Laser: Blue, Green, Yellow
Target Species: Human
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Isotype: IgG1
Order number: BG628653

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Quantity 100 tests Immunogen Human thymocytes Specifity The mouse monoclonal antibody... more
CyFlow™ CD1c PE
Quantity100 tests
ImmunogenHuman thymocytes
SpecifityThe mouse monoclonal antibody L161 recognizes CD1c antigen, a 43 kDa type I glycoprotein associated with β2-microglobulin. It is expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells, B and some T cells.
Background InformationCD1c (R7, BDCA1) together with CD1a andCD1b, belongs to group 1 of CD1 antigens. These non-classical MHC-like glycoproteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. The trafficking routes of the particular CD1 types differ and correspond to their ability to bind and present different groups of antigens. CD1c is unique in its ability to present e.g. mycobacterial phosphoketides and polyisoprenoids. CD1c is the only CD1 isoform that has been shown to interact both with α/β and γ/δ T cells.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Available Technical Data Sheets

Specific References

| Todate A, Chida K, Suda T, Imokawa S, Sato J, Ide K, Tsuchiya T, Inui N, Nakamura Y, Asada K, Hayakawa H, Nakamura H: Increased numbers of dendritic cells in the bronchiolar tissues of diffuse panbronchiolitis. Am·J·Respir·Crit·Care·Med. 2000·Jul; 162(1):148‑53. <·PMID:·10903234·> | Briken V, Jackman RM, Watts GF, Rogers RA, Porcelli SA: Human CD1b and CD1c isoforms survey different intracellular compartments for the presentation of microbial lipid antigens. J·Exp·Med. 2000·Jul·17; 192(2):281‑8. <·PMID:·10899914·> | del C Salamone M, Mendiguren AK, Salamone GV, Fainboim L: Membrane trafficking of CD1c on activated T cells. J·Leukoc·Biol. 2001·Oct; 70(4):567‑77. <·PMID:·11590193·> | Gutzeit C, Raftery MJ, Peiser M, Tischer KB, Ulrich M, Eberhardt M, Stockfleth E, Giese T, Sauerbrei A, Morita CT, Schönrich G: Identification of an important immunological difference between virulent varicella‑zoster virus and its avirulent vaccine: viral disruption of dendritic cellinstruction. J·Immunol. 2010·Jul·1; 185(1):488‑97. <·PMID:·20525895·> | Scharf L, Li NS, Hawk AJ, Garzón D, Zhang T, Fox LM, Kazen AR, Shah S, Haddadian EJ, Gumperz JE, Saghatelian A, Faraldo-Gómez JD, Meredith SC, Piccirilli JA, Adams EJ: The 2.5 Å structure of CD1c in complex with a mycobacterial lipid reveals an open groove ideally suited for diverse antigen presentation. Immunity. 2010·Dec·14; 33(6):853‑62. <·PMID:·21167756·>