CyFlow™ CD3 act. Purified

CyFlow™ CD3 act. Purified
Antigen: CD3 (activation epitope)
Clone: APA1/1
Application: Flow cytometry, Immunocytochemistry, Immunohistochemistry (frozen sections), Immunoprecipitation, Western blot
Format/Fluorochrome: Purified
Target Species: Human, Mouse
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Isotype: IgG1
Order number: CF594139

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

HLDA Workshop HLDA III—WS Code T 126 Concentration Unit mg/mL Concentration 1... more
CyFlow™ CD3 act. Purified
HLDA WorkshopHLDA III—WS Code T 126
Concentration Unitmg/mL
Quantity0.1 mg
ImmunogenPurified human CD3 proteins isolated from thymus
SpecifityThe mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 ε. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation
Background InformationCD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 γ, CD3 δ, CD3 ε and CD3 ζ. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Alarcón B, Ley SC, Sánchez-Madrid F, Blumberg RS, Ju ST, Fresno M, Terhorst C: The CD3‑gamma and CD3‑delta subunits of the T cell antigen receptor can be expressed within distinct functional TCR/CD3 complexes. EMBO·J. 1991·Apr; 10(4):903‑12. <·PMID:·1826255·> | Delgado P, Fernández E, Dave V, Kappes D, Alarcón B: CD3delta couples T‑cell receptor signalling to ERK activation and thymocyte positive selection. Nature. 2000·Jul·27; 406(6794):426‑30. <·PMID:·10935641·> | Torres PS, Alcover A, Zapata DA, Arnaud J, Pacheco A, Martín-Fernández JM, Villasevil EM, Sanal O, Regueiro JR: TCR dynamics in human mature T lymphocytes lacking CD3 gamma. J·Immunol. 2003·Jun·15; 170(12):5947‑55. <·PMID:·12794121·> | Gil D, Schrum AG, Alarcón B, Palmer E: T cell receptor engagement by peptide‑MHC ligands induces a conformational change in the CD3 complex of thymocytes. J·Exp·Med. 2005·Feb·21; 201(4):517‑22. <·PMID:·15728235·> | Risueño RM, Gil D, Fernández E, Sánchez-Madrid F, Alarcón B: Ligand‑induced conformational change in the T‑cell receptor associated with productive immune synapses. Blood. 2005·Jul·15; 106(2):601‑8. <·PMID:·15790785·> | Rieux-Laucat F, Hivroz C, Lim A, Mateo V, Pellier I, Selz F, Fischer A, Le Deist F: Inherited and somatic CD3zeta mutations in a patient with T‑cell deficiency. N·Engl·J·Med. 2006·May·4; 354(18):1913‑21. <·PMID:·16672702·> | Risueño RM, van Santen HM, Alarcón B: A conformational change senses the strength of T cell receptor‑ligand interaction during thymic selection. Proc·Natl·Acad·Sci·USA. 2006·Jun·20; 103(25):9625‑30. <·PMID:·16766661·> | Wong AP, Dutly AE, Sacher A, Lee H, Hwang DM, Liu M, Keshavjee S, Hu J, Waddell TK: Targeted cell replacement with bone marrow cells for airway epithelial regeneration. Am·J·Physiol·Lung·Cell·Mol·Physiol. 2007·Sep; 293(3):L740‑52. <·PMID:·17616650·> | Tailor P, Tsai S, Shameli A, Serra P, Wang J, Robbins S, Nagata M, Szymczak-Workman AL, Vignali DA, Santamaria P: The proline‑rich sequence of CD3epsilon as an amplifier of low‑avidity TCR signaling. J·Immunol. 2008·Jul·1; 181(1):243‑55. <·PMID:·18566390·>