CyFlow™ CD3 zeta Purified

CyFlow™ CD3 zeta Purified
Antigen: CD3 ζ (Phospho-Tyr142)
Clone: EM-54
Application: Flow cytometry, Western blot
Format/Fluorochrome: Purified
Target Species: Human, Mouse
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Isotype: IgG1
Order number: BQ894445

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Immunogen CP1 (pTyr142)... more
CyFlow™ CD3 zeta Purified
Concentration Unitmg/mL
Quantity0.1 mg
ImmunogenCP1 (pTyr142) - KLH
SpecifityThe mouse monoclonal antibody EM-54 recognizes phosphorylated tyrosine 142 of CD3 ζ chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
Background InformationCD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3γ, CD3 δ, CD3ε and CD3ζ. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Dong S, Corre B, Nika K, Pellegrini S, Michel F: T Cell Receptor Signal Initiation Induced by Low-Grade Stimulation Requires the Cooperation of LAT in Human T Cells. PLoS·One. 2010·Nov·30; 5(11):e15114. <·PMID:·21152094·>